Impact PCR-based Alternative to MAP Testing


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IMPACT: PCR-based Alternative to MAP testing

The Infectious Microbe PCR AmplifiCation Test, or IMPACT, is a panel of PCR assays that detects murine pathogens in biological samples as an alternative to the Mouse Antibody Production (MAP) test. Contamination of biological specimens, such as cell lines, hybridomas and tumor cells, with rodent pathogens can result in devastating outbreaks of disease in laboratory animals implanted with these materials and confounding and deleterious effects on tissue culture-based experiments. The traditional method for testing biological specimens for murine pathogens has been the Mouse Antibody Production (MAP) test. The major disadvantage of MAP testing is the 6 to 8 weeks required to get results. Comparison of IMPACT results with MAP testing results for representative DNA and RNA viruses indicated that the sensitivity of the IMPACT was equal or greater than that of MAP testing. Turnaround time for IMPACT results is usually 10 business days.

IMPACT Profiles - Mice

IMPACT Profile I (18 Agent Tests)	IMPACT Profile II (15 Agent Tests)	IMPACT Proifle III (13 Agent Tests)	IMPACT Profile IV (7 Agent Tests)
Fee = $450	Fee = $400	Fee = $350	Fee = $225
Mycoplasma spp.	Mycoplasma spp.	Mycoplasma spp.	Mycoplasma spp.
Mouse norovirus	Mouse norovirus	Mouse norovirus	Mouse norovirus
Sendai virus	Sendai virus	Sendai virus	Sendai virus
Mouse hepatitis virus	Mouse hepatitis virus	Mouse hepatitis virus	Mouse hepatitis virus
Pneumonia virus of mice	Pneumonia virus of mice	Pneumonia virus of mice	Pneumonia virus of mice
Mice minute virus	Mice minute virus	Mice minute virus	Mice minute virus
Mouse parvovirus	Mouse parvovirus	Mouse parvovirus	Mouse parvovirus
Theiler's murine encephalomyelitis virus	Theiler's murine encephalomyelitis virus	Theiler's murine encephalomyelitis virus	Theiler's murine encephalomyelitis virus
Reovirus3	Reovirus3	Reovirus3
Rotavirus	Rotavirus	Rotavirus
Ectromelia	Ectromelia	Ectromelia
Lymphocytic choriomeningitis virus	Lymphocytic choriomeningitis virus	Lymphocytic choriomeningitis virus
Polyoma	Polyoma	Polyoma
K virus	K virus	K virus
Mouse adenovirus	Mouse adenovirus
Cytomegalovirus	Cytomegalovirus
Lactate dehydrogenase-elevating virus
Mouse thymic virus
Hantaan

IMPACT Profiles - Rats

IMPACT Profile V (15 Agent Tests)	IMPACT Profile VI (11 Agent Tests)
Fee = $400	Fee = $325
Mycoplasma spp.	Mycoplasma spp.
Pneumonia virus of mice	Pneumonia virus of mice
Kilham's rat virus	Kilham's rat virus
Toolan's H1	Toolan's H1
Rat Parvovirus	Rat Parvovirus
Lymphocytic choriomeningitis virus	Lymphocytic choriomeningitis virus
Rat Cytomegalovirus	Rat Cytomegalovirus
Lactate dehydrogenase-elevating virus	Lactate dehydrogenase-elevating virus
Sendai	Sendai
Rat Coronavirus	Rat Coronavirus
Sialodacryoadenitis virus	Sialodacryoadenitis virus
Seoul
Mouse adenovirus
Reovirus3
Theiler's murine encephalomyelitis virus

Sample Collection and Preparation for Shipping

IMPACT:To test biological specimens or cultured cells by the IMPACT, send two 2ml cryovials of each sample with 1 x 107 cells/vial in 1 ml of growth media or phosphate-buffered saline to our laboratory on a minimum of 2 kg of dry ice by overnight courier.

Ascites fluids: Ascites fluids for PCR evaluation may be prepared in one of two ways. Freshly collected ascites fluid should be shipped cooled (not frozen) with wet ice in a styrofoam container by overnight service. Because of the limited number of cells that may be present in ascites, we recommend that 10 ml of fluid be submitted. Alternatively, cells in the fluids can be pelleted by centrifugation at 10,000 x g for 10 minutes, the supernatant removed, and the cell pellet frozen. The pelleted sample should be shipped on dry ice in a styrofoam container by an overnight carrier that handles dry ice shipments.

Tissue or cellular samples: Collection of tissues or cells for PCR evaluation should be performed aseptically to prevent inadvertent contamination of the samples. Each cellular sample should be placed in individually labeled sterile containers. When collecting samples, instruments used to collect the samples should be replaced between samples. Alternatively, instruments can be wiped clean and immersed in a dilute bleach (10%) solution for 10 minutes prior to reuse. Tissue samples should be frozen and shipped on dry ice in a styrofoam container by an overnight carrier service that handles dry ice shipments.

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06/19/2008