Proper tissue fixation is essential for accurate histopathologic evaluation. Ten-percent neutral buffered formalin is the tissue fixative of choice for routine H&E staining, with alcohol or formalin substitutes also acceptable. Tissues should be placed in a 10:1 volume of fixative to tissue immediately after dissection. Ideally, solid tissues should be no thicker than 5 mm, with larger tissues incised to allow penetration of fixative. Formalin infusion or flushing is recommended for lung and intestinal samples, especially for rodents.
Samples must be submitted in containers that are adequately sized, leak proof, and properly labeled. Multiple tissues from the same animal may be placed in one container. Submission of tissues from control animals is strongly recommended to ensure accurate interpretation of experimentally induced lesions. Histology slides prepared by other histotechnology laboratories may also be submitted for histopathologic interpretation.
A concise complete history is vital for the pathologist to provide you with the most meaningful interpretation of the submitted specimens. Indicate relevant clinical signs, manipulations, procedures, experiments, etc. in the "Comments/History/Special Instructions" section of the submission form. Identification of control animals is suggested, although "blind" interpretation will be performed if requested.
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